提取mRNA最后步骤为什么要在干冰上冻一下参考：1.Add10μLglycogen,30μLof2Msodiumacetate,600μL100%ethanol,andmix.2.Freezeondryiceuntilsolid(orstoreat-80°C).3.Thawandcentrifugeat16,000xg,15minutes,+4°C.4

Question

提取mRNA最后步骤为什么要在干冰上冻一下
参考：
1.Add 10 μL glycogen,30 μL of 2 M sodium acetate,600 μL 100%
ethanol,and mix.
2.Freeze on dry ice until solid (or store at -80°C).
3.Thaw and centrifuge at 16,000 x g,15 minutes,+4°C.
4.Remove all the ethanol.
5.Resuspend the mRNA pellet in 1-10 μL Elution Buffer.
6.Determine the concentration of the mRNA.
7.Store mRNA at -80°C or use immediately.

Answer 1

他意思是如果你不是马上用的话要用低温保存；马上用的话就不用冻.冻只是为了保存而已.