英语翻译AdipocytesweredifferentiatedfollowingthemethodsofXieetal.(Xieetal.,2006).Briefly,pre-adipocyteswereculturedtoconflu-enceusingpre-adipocyteculturemedium(90%Dulbecco’smodifiedEagle’smedium[DMEM

Question

英语翻译
Adipocytes were differentiated following the methods of Xie et al.(Xie et al.,2006).Briefly,pre-adipocytes were cultured to conflu-ence using pre-adipocyte culture medium (90% Dulbecco’s modified Eagle’s medium [DMEM] with high glucose supplementedwith 10% bovine serum and antibiotics).Differentiation in cellswas initiated with freshly-prepared adipocyte differentiation ini-tiation medium containing high glucose DMEM supplementedwith 10% fetal bovine serum,antibiotics,1 M dexamethasone,0.5mM isobutylmethylxanthine,and 1 g/ml insulin.After 4 d of
initiation,the culture medium was replaced with the freshly-prepared progression medium containing high glucose DMEM supplemented with 10% fetal bovine serum,antibiotics,and 1 g/ml insulin.After 4 days of progression,the medium was changed to
adipocyte culture medium and was changed every two days until experimentation.

Answer 1

通常,脂肪细胞的分化采用谢氏分离法（参见Xie et al., 2006）.谢氏分离法简单陈述如下：前成脂肪细胞（未成形的脂肪细胞）在其培养介质中培养,分裂并聚集成团（培养介质为90%的达尔伯克改良伊格尔培养基混合10%的牛血清与抗体的混合物）.
脂肪细胞分化的初始阶段使用新鲜（新制）的脂肪细胞分化初始介质,成分为高糖分的达尔伯克改良伊格尔培养基混合10%含量的标准胎牛血清、抗体、1摩尔的德萨美松,0.5毫摩的异丁基甲基黄嘌呤以及浓度为1克每毫升的胰岛素液.
4天的初始阶段过后,初始培养介质需要换成新鲜的发展介质（这些只是形象地这么叫,因为初始准备"initiation”之后就是发展"progression",可以无视,重要的是后面的成分）,主成分依然是高糖分的达尔伯克改良伊格尔培养基,补料为10%的标准胎牛血清、抗体以及浓度为1克每毫升的胰岛素液（其实就是去掉了1摩尔的德萨美松和0.5毫摩的异丁基甲基黄嘌呤）.
4天的发展阶段过后,介质需要再次更换,换为脂肪细胞正式培养介制,之后这种介质每两天都要更换一次,直到可用于实验为止.
以上为翻译内容,树的儿子为您解答,希望能够帮到楼主,