求英语化学翻译大神~~TheassayprocedurereportedbyKumar,Paul,&Sharma,2011,wasusedwithslightmodification(Kumaretal.,2011).Blankgoldnanoparticlesweredispersedinnormalphysiologicalsaline(0.9

Question

求英语化学翻译大神~~
The assay procedure reported by Kumar, Paul, & Sharma, 2011,was used with slight modification (Kumar et al., 2011). Blank gold nanoparticles were dispersed in normal physiological saline (0.9%, w/v NaCl). Varying concentration (50–200 g/ml) of thedispersion were added into red blood cells suspension (2%, v/v),mixed well and incubated at 37 ◦C. After 1 h, the samples were centrifuged at 3000 rpm for 5 min and absorbance of supernatant was measured at 540 nm spectrophotometrically. The supernatant absorbance of RBC suspension treated with distilled water and normal saline were taken as standard and control, respectively. The % hemolysis was calculated as: % hemolysis = (Asample − Acontrol)/(Astandard− Acontrol) × 100.

Answer 1

Kumar, Paul, 和 Sharma在2011年对实验进行了略微的修改。将空白金纳米粒子分散在普通的生理盐水（质量体积比为0.9%的NaCl）中。将不同浓度的悬浊液（50~200g/mL之间）加入到红细胞悬浊液（体积比2%）中，混合均匀并在37℃保温。1小时后将混合液离心(3000转/分)5分钟。取上清液，在分光光度计中测定540nm发射光的吸光度。分别将采用蒸馏水和普通生理盐水处理的红细胞悬浊液在540nm发射光下测定的吸光度作为标准组和控制组。溶血分数采用以下计算公式：
溶血分数%=（样品吸光度-控制组吸光度）/（标准组吸光度-控制组吸光度）× 100