英语翻译Fortheanalysis,50mlofeachextractwastransferredwithapipetteintoa100-mlround-bottomflaskandthesolventwasdistilledoffinvacuumonarotorevaporator(Watterbath-480,Büchi,Switzerland)atawaterbathtemperaturewithin60

Question

英语翻译
For the analysis,50 ml of each extract was transferred
with a pipette into a 100-ml round-bottom flask and the solvent
was distilled off in vacuum on a rotor evaporator
(Watterbath-480,Büchi,Switzerland) at a water bath temperature
within 60 – 90°C.The dry residue was dissolved in
5 ml of chloroform and this solution was analyzed by TLC
on 10 \x06 10 cm Merck G-60 plates.Three samples were applied
with a micropipette onto the start line (0.07 ml of sample
solution and 0.01 ml of a 25% aqueous ammonium hydroxide
solution) and the plate was dried for 20 min in air.
Then the plate was placed into a TLC chamber (presaturated
for 1 h) with a chloroform – ethanol (90 :10) mixture and
chromatographed in the ascending mode at 25°C.
The bands containing sanguinarine and chelerythrine
(colored orange and yellow,Rf 0.6) were quantitatively
transferred into 50-ml flasks with ground-glass joints and
10 ml of ethanol acidified with 1% H2SO4 added.The solutions
were stirred for 5 min,transferred into centrifuge tubes,
and centrifuged for 20 min.The quantitative analysis for alkaloids
was performed (according to VFS 42-2666–89) by
spectrophotometry at 452 nm (Hitachi EP-3T spectrophotometer).

Answer 1

用移液器将每个提取物的50毫升溶液转移到100毫升的圆底瓶中,有机溶剂在真空下由旋转蒸发仪（型号是水浴- 480 ,büchi ,瑞士）旋掉,水浴温度保持在60 -9 0摄氏度.旋干后的产物溶解在5毫升的氯仿中,此溶液在10-10 cm Merck G-60 TLC板分析.样品用微样进样器取（0.07毫升样品和0.01毫升的25%浓度的氢氧化铵溶液）点至硅胶板的起始位置,并在空气中自然干燥20分钟.然后将此硅胶板放入TLC展缸中（事先气相饱和一小时）,展开剂为氯仿比乙醇90：10,在室温25摄氏度下展开.色带中包含血根碱和白屈菜赤碱（颜色为橙色和黄色,Rf值为0.6）分别转移至50毫升单口瓶并加入又1%硫酸酸化的乙醇10毫升.溶液搅拌5分钟,转移至离心管并离心20分钟.生物碱的定量分析完成（根据VFS 42-2666–89）通过光度法在452纳米分析得到（日立的EP - 3T分光光度计）.