0.1M的2-吗啉乙磺酸钠缓冲液怎么配

Question

0.1 M 的2-吗啉乙磺酸钠缓冲液怎么配

Answer 1

Say you want to make 1 L of 100 mM MES at pH 6.0.The MW of MES (free acid) is 195.2.1 L at 0.1 mole/L requires 0.1 mole of MES or 19.5 g of MES.Put 19.5 g of MES in something like 500 ml of water,put in a pH electrode (the starting pH will be something below 4.0 probably) and titrate to pH 6.0 with either 1 N or 10 N NaOH.Once you’ve got the solution to the correct pH,transfer it to a graduated cylinder or volumetric flask and dilute it to a final volume of 1 L and you’re done.Different volumes or different desired concentrations of MES will require the use of different amounts os solid MES,but the process will be the same.