人正常乳腺细胞（MCF-10A）如何培养,培养基如何配制?

Question

人正常乳腺细胞（MCF-10A）如何培养,培养基如何配制?

Answer 1

Components for Medium recipes for MCF-10A ER-Src cells
DMEM/F12 (Invitrogen No.11039-021)
Horse serum (Invitrogen No.16050-122)
Pen/Strep (100X solution,Invitrogen No.15070-063)
EGF (Peprotech,1 mg):Resuspend at 100 ug/ml in sterile ddH2O.Store aliquots at -20°C.
Hydrocortisone:(Sigma No.H-0888,1-g bottles) Resuspend at 1 mg/ml in 200-proof ethanol and store
aliquots at -20°C.
Cholera toxin:(Sigma No.C-8052,2-mg vials) Resuspend at 1 mg/ml in sterile ddH2O and allow to
reconstitute for about 10 min.Store aliquots at 4°C.
Insulin (Sigma No.I-1882,100-mg vials) Resuspend at 10 mg/ml in sterile ddH2O containing 1% glacial
acetic acid.Shake solution and allow 10–15 min to reconstitute.Store aliquots at -20°C.