英语翻译1.AssembleFFPEsectionsequivalentto≤35mgunsectionedcore2.Add1mL100%xylene,mix,andincubatefor3minat50°C3.Centrifugefor2minatmaximumspeed,anddiscardthexylene4.Washthepellettwicewith1mL100%ethanol5.AddD

Question

英语翻译
1.Assemble FFPE sections equivalent to ≤ 35 mg unsectioned core
2.Add 1 mL 100% xylene,mix,and incubate for 3 min at 50°C
3.Centrifuge for 2 min at maximum speed,and discard the xylene
4.Wash the pellet twice with 1 mL 100% ethanol
5.Add Digestion Buffer and Protease
6.Incubate at 50°C/80°C (RNA) or at 50°C (DNA)
7.Prepare Isolation Additive/ethanol mixture
8.Add Isolation Additive/ethanol and mix
9.Pass the mixture through a Filter Cartridge
10.Wash with 700 μL of Wash 1
11.Wash with 500 μL of Wash 2/3,and then centrifuge to remove residual fluid
12.Add DNase or RNase mix to each Filter Cartridge and incubate for 30 min
13.Wash with 700 μL of Wash 1
14.Wash twice with 500 μL of Wash 2/3,then centrifuge to remove residual fluid
15.Elute with 60 μL Elution Solution or nuclease-free water at room
Temp.

Answer 1

1.配备约相当于35mg unsectioned core的FFPE物体.
2.添加1毫升浓度100%甲笨后混合均匀,安放在50度的环境里三分钟.
3.以最大的转速分离出甲笨,时间设定为二分钟.
4.用1 mL 浓度100%乙醇清洗小球.
5.添加消化缓冲溶液与蛋白酶
6.RNA可放于 50°C/80°C中培养.DNA放于50°C中.
7.准备分离添加物即乙醇混合物
8.加入上述溶液混合.
9.把混合溶液加入过滤筒中.10.用上述第一条的700 μL溶液清洗.
11.用上述第二条或三条的700 μL溶液清洗,然后用离心机清除多余的液体.
12.加入DNA或RNA混合物到各分离器中,放置30分钟.
13.用700 μL 的第一项溶液清洗.
14.用500 μL 的第二项或第三项溶液清洗,分离出多余的液体.
15.在恒温环境中用60ul稀析液体或无核酸酶水稀析.