英语翻译Followingstimulation,cellswerewashedoncewithphosphatebufferedsaline(PBS)andsolubilizedinlysisbuffercontaining20mMTris-HCl,pH7.4,0.8%TritonX-100,150mMNaCl,2mMEDTA,10%glycerol,100μMphenylmethylsulfonylfluoride(PMSF),

Question

英语翻译
Following stimulation,cells were washed once with phosphate
buffered saline (PBS) and solubilized in lysis buffer containing
20mM Tris-HCl,pH 7.4,0.8% Triton X-100,150mM NaCl,2mM
EDTA,10% glycerol,100 μM phenylmethylsulfonyl fluoride
(PMSF),5 μg/mL aprotinin,and 5 μg/mL leupeptin [8].The cell lysates
were immunoblotted with anti-renin,anti-ACE,or anti-
GAPDH antibodies.

Answer 1

亲.这都是化学的专业术语 没法给你全翻译因为这貌似是个lab report...phosphate磷酸 PBS是缓冲盐水 PH这些你应该知道是什么吧就是ph值 酸碱的那些.NaCl - Sodium Chloride 是一个化学术语.phenylmethylsulfonyl fluoride 是一个titration 之后的现象..（抱歉因为我学的是英文版的.所以相对中文不太了解.你可以问问专业学化学的） 就只能翻译这么多了