英语翻译BacillussubtilisnattostrainwasisolatedfromcommercialnattoavailableintheAustralianmarket.Bacterialcellswerecultivatedinaliquidcultureconstituting0.5%(w/v)peptone,0.5%(w/v)glucoseand0.05%(w/v)yeastextractfor@

Question

英语翻译
Bacillus subtilis natto strain was isolated from commercial natto available in the Australian market.Bacterial cells were cultivated in
a liquid culture constituting 0.5% (w/v) peptone,0.5% (w/v)
glucose and0.05%(w/v) yeast extract for five days before streaking
on tryptic soy agar plates for aseptic spore genesis.Plates were
scrapped after five days and harvested cells were suspended in a
0.9% (w/v) NaCl solution.The spore suspension was kept in a
heated water bath (808C,30 min) to kill the residual vegetative
cells,centrifugedat3000 rpm for 10 min to remove the cell debris,
diluted using 0.9% (w/v) NaCl solution to obtain the standard
spore solution (5.2 \3 0.5 \4 1010 spores/mL).

Answer 1

纳豆芽孢杆菌菌株是从澳大利亚market.Bacterial细胞提供商业纳豆中分离培养在构成0.5％的液体培养物（重量/体积）蛋白胨,0.5％（重量/体积）划线前葡萄糖和0.05％（重量/体积）酵母提取物五天对胰蛋白酶大豆琼脂平板上无菌孢子起源.板五天后报废和回收的细胞在被停职0.9％（重量/体积）NaCl溶液.的孢子悬浮液保持在恒温水浴锅（808C,30分钟）,以杀死残留的营养centrifugedat3000 rpm离心10分钟以除去细胞碎片,使用0.9％（重量/体积）NaCl的溶液中,得到的标准稀释孢子溶液（5.2≤0.5≤1010孢子/毫升）