帮我修改一下自己写的英文3急用,1,Post-harvestedgrapeberriesoncoldstoragehastwokindsofdifferentgraymoldsymptoms:onesporulateongrapeberriesrapidlyanddevelopalargeamountofspores,causethegrapedecayrapidly,theot

Question

帮我修改一下自己写的英文3 急用,
1,Post-harvested grape berries on cold storage has two kinds of different gray mold symptoms:one sporulate on grape berries rapidly and develop a large amount of spores,cause the grape decay rapidly,the other develop no spores on grape berries,only mycelium.
5,Use species-specific primers to amplify B.cinerea species-specific fragment showed that only EXFG Ⅲ has this fragment with differences in sequence among strains,confirmed the morphological classification result that EXFG Ⅲ is typical B.cinerea.Further amplification of the 1171bp fragment of B.cinerea Bc-hch gene show that EXFG Ⅱ and EXFG Ⅲ have this fragment,HhaI digestion show no difference in sequence.These results show that EXFG Ⅱ and EXFG Ⅲ may be two different subspecies in B.cinerea.EXFG Ⅰ has no B.cinerea specific sequence,and may be other species of the genus Botrytis.
6,Analysis the differences in rDNA-ITS sequence of 3 groups,the result showed that the rDNA-ITS sequence of 3 groups only different in one or two bases,and could not identify the strains of 3 groups base on rDNA-ITS sequence analysis.ISSR(Inter-Simple Sequence Repeat) cluster analysis show the genetic differences between strains from 3 groups.the results show that EXFG Ⅰhad a genetic distance of 0.89 compared with two other groups.Genetic distance between EXFG Ⅱand B.cinerea group EXFG Ⅲ was 0.74.The results show that srains of 3 groups have large genetic differences,and confirmed the division of groups by morphological classification.
7,In order to establish a method for rapid detection of the highly pathogenic group EXFG Ⅱ,we have developed a pair of specific molecular markers GBOT-20±by the use of the SCAR technology.GBOT-20±can be used for the specific detection and quantitative analysis of grapes gray mold EXFG Ⅱ on grape plant or post-harvested grape berries.
To sum up,gray mold pathogens on table grape can be divided into 3 groups.EXFG Ⅱ is different from the previous reported gray mold pathogen (B.cinerea) in morphological character,sporulation time and symptoms,as well as genetic characteristic.EXFG Ⅱ have high pathogenicity and infect a wide range of hosts and sporulate rapidly on grape berries.It is a very dangerous new groups.EXFG Ⅰmay be other species of the genus Botrytis.EXFG Ⅲ is typical gray mold pathogen (Botrytis cinerea Pers.:Fr.) previous reported.This study provide useful reference for the classification of species or subspecies related to B.cinerea,and set an important basis for adequate control measures.

Answer 1

1,Post-harvested grape berries on cold storage manifest two kinds of different gray mold symptoms:the first sporulate on grape berries rapidly and develop a large amount of spores,cause the grape to decay rapidly,the second develop no spores on grape berries,only mycelium.
5,Using species-specific primers to amplify B.cinerea species-specific fragments showed that only EXFG Ⅲ displays this fragment with differences in sequence among strains,and confirms the morphological classification result that EXFG Ⅲ is a typical B.cinerea.Further amplification of the 1171bp fragment of B.cinerea Bc-hch gene show that EXFG Ⅱ and EXFG Ⅲ have this fragment,while HhaI digestion shows no difference in sequence.These results show that EXFG Ⅱ and EXFG Ⅲ may be classifeid as two different subspecies of B.cinerea.EXFG Ⅰ has no B.cinerea specific sequence,and may be just another species of the genus Botrytis.
6,The results of the analysis of the differences in rDNA-ITS sequence of 3 groups,show that the rDNA-ITS sequence of 3 groups differ only in one or two bases,and could not identify the strains of 3 groups base on rDNA-ITS sequence analysis.ISSR(Inter-Simple Sequence Repeat) cluster analysis show the genetic differences between strains from 3 groups.The results show that EXFG Ⅰhad a genetic distance of 0.89 compared with two other groups.Genetic distance between EXFG Ⅱand B.cinerea group EXFG Ⅲ was 0.74.The results show that strains of 3 groups have large genetic differences,and confirmed the classification of groups on the basis of morphological criteria.
7,In order to establish a method for rapid detection of the highly pathogenic group EXFG Ⅱ,we have developed a pair of specific molecular markers GBOT-20±by the use of the SCAR technology.GBOT-20±can be used for the specific detection and quantitative analysis of grapes gray mold EXFG Ⅱ on grape plant or post-harvested grape berries.
To sum up,gray mold pathogens on table grape can be divided into 3 groups.EXFG Ⅱ is different from the above mentioned gray mold pathogen (B.cinerea) in its morphology,sporulation time and symptoms,as well as genetic characteristics.EXFG Ⅱ have high pathogenicity and infect a wide range of hosts and sporulate rapidly on grape berries.It is a very dangerous new group of plant infectants.EXFG Ⅰ is possibly another species of the genus Botrytis.EXFG Ⅲ is a typical gray mold pathogen (Botrytis cinerea Pers.:Fr.) as previously mentioned.This study provides useful reference for the classification of species or subspecies related to B.cinerea,and provides an important basis for adequate control measures.